Base Editing
Base editing is redefining what’s possible in functional genomics and therapeutic discovery. Unlike traditional CRISPR-Cas9 genome editing—which creates double-strand breaks—base editors enable precise, single-nucleotide changes without disrupting genomic integrity. This technology makes it possible to model disease-associated mutations, correct pathogenic variants, and systematically explore the functional impact of point mutations across the genome.
At Atlas Biotech, we offer complete base editor experimental services—from pooled library screening to bespoke single-guide cell line generation—providing a powerful toolkit for precision functional genomics and drug discovery.
Why Base Editing?
Base editors combine the targeting flexibility of CRISPR with the precision of chemical modification:
Single-nucleotide resolution – Create defined point mutations without indels.
Higher editing efficiency – Minimized byproducts and off-target effects.
Physiologically relevant models – Replicate patient mutations for functional studies.
Broad applicability – From disease modeling to therapeutic target validation.
Our Base Editing Capabilities
1. Pooled Base Editor Library Screening
High-throughput pooled screening allows the parallel introduction of thousands of precise mutations in a single experiment:
Custom or genome-wide libraries targeting coding regions, regulatory elements, or known mutation hotspots.
Positive or negative selection assays to identify mutations that alter phenotype, survival, or drug sensitivity.
NGS-based readouts with robust bioinformatics pipelines for hit identification and functional annotation.
Applications include:
Saturation mutagenesis for functional mapping of proteins.
Screening for resistance or sensitivity mutations in targeted therapies.
Exploring genotype–phenotype relationships at single-nucleotide resolution.
2. Single-Guide Cell Line Generation
For focused, hypothesis-driven studies, we generate isogenic cell lines with precise base edits:
Knock-in of clinically relevant variants for disease modeling.
Mutation correction to validate therapeutic editing strategies.
Reporter integration for assay development and real-time monitoring.
Compatible with multiple cell types, including primary and hard-to-transfect lines.
Applications include:
Mechanistic studies of specific mutations.
Drug mechanism-of-action validation.
Biomarker discovery and patient stratification assays.
Workflow & Data Integration
Our team provides end-to-end support:
Project design – Select editing strategy, choose base editor variant, and define target sites.
Editing & validation – Optimize delivery, confirm edits via deep sequencing.
Phenotypic assays – Measure the biological impact of introduced mutations.
Bioinformatic analysis – From variant calling to pathway-level insights.
We integrate results with complementary datasets—such as RNA-seq, proteomics, or CRISPR knockout/activation screens—to deliver a complete picture of mutation impact.
Why Partner with Us
Expertise in both pooled and single-guide approaches
Flexible experimental design to match your research goals
Validated workflows for high editing efficiency and reproducibility
Comprehensive reporting with actionable insights for discovery programs
Get Started
Base editing opens the door to precise, scalable, and biologically relevant functional genomics experiments. Whether you need a large-scale pooled library screen or a custom single-guide engineered cell line, Atlas Biotech delivers the technology and expertise to bring your vision to life.
Contact us to discuss your project and explore how base editing can accelerate your discoveries.