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Ba/F3 is an interleukin-3 (IL-3)-dependent murine pro-B cell line widely used in cancer biology, immunology, and signal transduction research. Originally derived from the bone marrow of a BALB/c mouse, Ba/F3 cells undergo apoptosis in the absence of IL-3, making them a powerful system for studying cytokine signaling, oncogene addiction, and growth factor receptor activity.
The Ba/F3 system is especially valuable for functional validation of oncogenic mutations: introducing mutant kinases (e.g., BCR-ABL, FLT3, JAK2, EGFR) into Ba/F3 cells allows for IL-3-independent growth, serving as a readout for constitutive activation and transformation. This makes Ba/F3 a gold-standard model for evaluating kinase inhibitors and resistance mutations in vitro.
Ba/F3 is an interleukin-3 (IL-3)-dependent murine pro-B cell line widely used in cancer biology, immunology, and signal transduction research. Originally derived from the bone marrow of a BALB/c mouse, Ba/F3 cells undergo apoptosis in the absence of IL-3, making them a powerful system for studying cytokine signaling, oncogene addiction, and growth factor receptor activity.
The Ba/F3 system is especially valuable for functional validation of oncogenic mutations: introducing mutant kinases (e.g., BCR-ABL, FLT3, JAK2, EGFR) into Ba/F3 cells allows for IL-3-independent growth, serving as a readout for constitutive activation and transformation. This makes Ba/F3 a gold-standard model for evaluating kinase inhibitors and resistance mutations in vitro.