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HEK293T cells are a derivative of the parental HEK293 cell line, stably transfected with the SV40 large T antigen. This modification enables episomal replication of plasmids containing the SV40 origin of replication, significantly increasing the yield of transiently expressed proteins or viral particles. HEK293T cells retain the high transfection efficiency and rapid growth characteristics of HEK293, with enhanced capabilities for transient gene expression and viral vector production.
Due to their ease of culture, robust growth, and compatibility with multiple transfection methods, HEK293T cells are a cornerstone of molecular biology and biotechnology workflows, especially in applications requiring high-level protein or virus production.
HEK293T cells are a derivative of the parental HEK293 cell line, stably transfected with the SV40 large T antigen. This modification enables episomal replication of plasmids containing the SV40 origin of replication, significantly increasing the yield of transiently expressed proteins or viral particles. HEK293T cells retain the high transfection efficiency and rapid growth characteristics of HEK293, with enhanced capabilities for transient gene expression and viral vector production.
Due to their ease of culture, robust growth, and compatibility with multiple transfection methods, HEK293T cells are a cornerstone of molecular biology and biotechnology workflows, especially in applications requiring high-level protein or virus production.