The Ba/F3 cell line has been used in the study of kinase activity for decades. This immortal pro-B murine cell line naturally requires interleukin-3 to grow, but can be made dependent on the expression of a transgene that activates proliferative signaling pathways, such as EGFR or ALK.
The Ba/F3 cell line has been used in the study of kinase activity for decades. This immortal pro-B murine cell line naturally requires interleukin-3 to grow, but can be made dependent on the expression of a transgene that activates proliferative signaling pathways, such as EGFR or ALK.