Deep Mutational Scanning
Deep Mutational Scanning (DMS) is a powerful functional genomics technology that systematically measures the effects of every possible amino acid change in a protein. By combining high-throughput mutagenesis, functional selection, and next-generation sequencing (NGS), DMS provides an unprecedented, quantitative map of how sequence variation impacts protein function, stability, and drug response.
At Atlas Biotech, we deliver end-to-end DMS services that help researchers:
Identify functional hotspots in therapeutic targets.
Predict the impact of patient mutations.
Uncover drug resistance mechanisms.
Guide rational drug design and precision medicine strategies.
Why Deep Mutational Scanning?
DMS enables researchers to move beyond individual mutation studies and into comprehensive protein-wide understanding:
Complete variant coverage – Analyze thousands to millions of variants in parallel.
Quantitative functional mapping – Link sequence changes to biochemical activity, binding affinity, or cellular phenotype.
Predictive insight – Anticipate mutation-driven drug resistance and escape pathways.
Precision medicine – Match therapeutic strategies to patient-specific variants.
Our DMS Capabilities
1. Library Design & Construction
We design and synthesize mutagenesis libraries that cover:
Single amino acid substitutions across the full protein sequence.
Domain-specific saturation mutagenesis for focused studies.
Patient-relevant mutation panels from clinical datasets.
2. Functional Selection Assays
Our platform supports a variety of functional readouts:
Binding & interaction assays – Quantify effects on target–ligand or protein–protein interactions.
Cell-based functional assays – Assess signaling, viability, or reporter activation.
Drug sensitivity/resistance profiling – Identify escape mutations under therapeutic pressure.
3. High-Throughput Sequencing & Analysis
NGS-based quantification of variant abundance pre- and post-selection.
Bioinformatic pipelines for calculating functional scores for each variant.
Structural and pathway-level mapping to contextualize results.
Applications of DMS
Our clients use DMS to:
Map drug resistance landscapes for small molecules (see our case study of imatinib resistance in CML here).
Identify essential residues for protein stability and activity.
Guide protein engineering for improved therapeutic properties.
Inform clinical decision-making with variant impact predictions.
Why Partner with Us
Customizable workflows for your target protein and desired readouts.
Integrated experimental & computational expertise to maximize data value.
High-throughput capacity for rapid turnaround on large datasets.
Regulatory-grade data reporting suitable for translational and preclinical programs.
Get Started
Deep Mutational Scanning delivers the most comprehensive view possible of how sequence variation affects protein function—empowering drug discovery, biomarker development, and precision therapy design.
Whether you’re mapping resistance pathways, engineering a better therapeutic, or interpreting patient mutations, Atlas Biotech has the technology, expertise, and insight to make it happen.
Contact us today to discuss your DMS project and learn how we can accelerate your research.